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1.
Pesqui. vet. bras ; 41: e06775, 2021. tab, graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1250484

ABSTRACT

Skin and cartilage have been the main source for the recovery of somatic cells to be used in conservation strategies in wild mammals. In this sense, an important step for the cryopreservation of these samples is to recognize the properties of the skin and cartilage. Thus, knowing that the skin may differ among species and aiming to contribute to the establishment of cryobanks, the study examined the differences in the ear skin and cartilage of wild rodents from South America, agouti (Dasyprocta leporina) and spix's yellow-toothed cavy (Galea spixii). Ultrastructural and quantitative methods were used to measure skin and cartilage thickness, density of collagen and elastic fibers, cell type number and distribution, and proliferative activity. Although ultrastructural analysis revealed a similar pattern between species, morphometric analysis of the skin and cartilage showed differences between agoutis and cavies regarding thickness of epidermis layers (corneum: 5.3±2.5μm vs. 3.9±0.6μm; intermediate: 16.4±6.2μm vs. 23.4±8.1μm; basal: 9.9±2.1μm vs. 4.8±0.5μm), dermis (183.1±44.0μm vs. 258.2±22.9μm), total skin (211.8±46.0μm vs. 290.3±23.7μm) and perichondrium (27.6±6.1μm vs. 10.5±1.8μm). A greater number of epidermal cells (61.7±15.2 vs. 24.8±7.6) and chondrocytes (32.7±9.0 vs. 27.5±4.7) were observed in agouti, while the cavy presented a greater number of melanocytes (12.6±4.7 vs. 29.9±6.2), keratinocytes (14.7±4.2 vs. 29.8±7.6), and fibroblasts (103.6±24.7 vs. 112.2±11.3). Moreover, a higher percentage of collagen fibers and proliferative activity was observed in the skin of cavies, when compared to the skin of agoutis. Therefore, there are differences between agouti and cavy for ear skin and cartilage, requiring the establishment of species-specific cryopreservation protocols.(AU)


A pele e cartilagem têm sido uma importante fonte de recuperação de células somáticas a serem utilizadas em estratégias de conservação em mamíferos silvestres. Nesse contexto, uma importante etapa para criopreservação é conhecer, inicialmente, as propriedades que compõem a pele e cartilagem. Sabendo, então, que a pele pode diferir-se entre espécies e com o objetivo de contribuir para o estabelecimento de criobancos, o estudo evidenciou as diferenças da pele e da cartilagem do pavilhão auricular apical de cutias (Dasyprocta leporina) e preás (Galea spixii) que são roedores silvestres presentes na América do Sul. Para tanto, métodos ultraestruturais e quantitativos foram utilizados para mensurar a espessura da pele e da cartilagem, densidade de fibras colágenas e elásticas, número e distribuição dos tipos celulares e atividade proliferativa. Embora as propriedades ultraestruturais em cutias e preás tenham se mostrado semelhantes, avaliações acerca da morfometria da pele e da cartilagem demonstrou diferenças, especialmente nas camadas epidérmicas (córnea: 5,3±2,5μm vs. 3,9±0,6μm; espinhosa: 16,4±6,2μm vs. 23,4±8,1μm; basal: 9,9±2,1μm vs. 4,8±0,5μm), derme (183,1±44,0μm vs. 258,2±22,9μm), pele total (211,8±46,0μm vs. 290,3±23,7μm) e pericôndrio (27,6±6,1μm vs. 10,5±1,8μm). Além disso, um número maior de células epidérmicas (61,7±15,2 vs. 24,8±7,6) e condrócitos (32,7±9,0 vs. 27,5±4,7) foram observados em cutias, enquanto em preás um maior número de melanócitos (12,6±4,7 vs. 29,9±6,2), queratinócitos (14,7±4,2 vs. 29,8±7,6) e fibroblastos (103,6±24,7 vs. 112,2±11,3) foram evidenciados. Ainda, em preás, uma maior porcentagem de fibras colágenas e da atividade proliferativa foram observadas quando comparadas a pele de cutias. Portanto, existem diferenças entre cutias e preás para pele e cartilagem do pavilhão auricular, exigindo desta forma um estabelecimento de protocolos de criopreservação específica para cada uma destas espécies.(AU)


Subject(s)
Animals , Rodentia/anatomy & histology , Ear Cartilage , Epidermal Cells , Animals, Wild/anatomy & histology , Cryopreservation , Elastic Tissue , Dasyproctidae
2.
Braz. j. biol ; 76(3): 645-655, tab, graf
Article in English | LILACS | ID: lil-785027

ABSTRACT

Abstract Considering the physiological importance and need of greater morphophysiological knowledge of adrenal glands, the aims of present study were compare the morphometric data between left and right adrenal of male and female; perform a histological, scanning and transmission electron microscopy study showing tissue constitution of glands; finally, in order to define the presence and correct site of the cytochrome P450c17 expression in adrenal glands, immunohistochemical study of this enzyme was performed in 18 adrenal glands (right n=9 and left n=9) of nine adult Galea spixii (four males and five females). Right adrenal was more cranially positioned than left adrenal; dimensions (weight, length and width) of right adrenal was larger than left adrenal; no differences between male and female body and adrenal measurements were found; the morphology of cells and different amounts of lipid droplets may be related to the different demands of steroid hormones production, related to each zone of the adrenal cortex; and, the cytochrome P450c17 immunolocalization in fasciculate and reticular zone may be related with synthesis of 17-hydroxy-pregnenolone, 17-hydroxy-progesterone, dehydroepiandrosterone or androstenedione.


Resumo Considerando a importância fisiológica e a necessidade de melhor conhecimento morfofisiológico das glândulas adrenais, os objetivos do presente estudo foram comparar dados morfométricos entre adrenais direitas e esquerdas de machos e fêmeas; realizar estudos histológicos e microscopia eletrônica de varredura e transmissão revelando a constituição tecidual das glândulas; finalmente, foi realizado estudo imunohistoquímico com objetivo de definir a presença e correto sítio de expressão da citocromo P450c17 nas glândulas adrenais em 18 glândulas adrenais (direita n=9 e esquerda n=9) de nove Galea spixii adultos (quatro machos e cinco fêmeas). A glândula adrenal direita estava posicionada mais cranialmente que a adrenal esquerda; as dimensões (peso, comprimento e largura) da glândula adrenal direita foram maiores que as da esquerda; não houve diferença entre as medidas corpóreas e das adrenais entre machos e fêmeas; a morfologia das células e diferentes quantidades de gotículas lipídicas podem estar relacionadas a diferentes demandas na produção de hormônios esteroides relacionados a cada zona do córtex adrenal; e, a imunolocalização da citocromo P450 na zona fasciculada e reticular pode estar relacionado com a síntese de 17-hidroxi-pregnenolona, 17-hidroxi-progesterona, dehidroepiandrosterona ou androstenediona.


Subject(s)
Animals , Male , Female , Rodentia/anatomy & histology , Adrenal Glands/anatomy & histology , Guinea Pigs
3.
Ciênc. rural ; 46(5): 871-875, May 2016. tab
Article in English | LILACS | ID: lil-777277

ABSTRACT

ABSTRACT: From May to September 2011, a total of 138 wild rodents of the Cricetidae family were collected in the cities of Anhembi, Bofete and Torre de Pedra, in São Paulo State. All animals were examined for the presence of ticks, which were collected and identified at species level in the laboratory by morphological examination (for adults, nymphs and larvae) and molecular biology, by sequencing of a fragment of the 16S mitochondrial rDNA (for larvae). A total of 47 ticks (21 larvae and 26 nymphs) were collected from rodents, identified as 21 larvae and 23 nymphs of Amblyomma ovale (collected on Oligoryzomys nigripes, Oligoryzomys flavescens, Sooretamys agouya and Nectomys squamipes), two nymphs of Ixodes schulzei (O. nigripes and O. flavescens) and an Amblyomma sculptum nymph in S. agouya. The overall prevalence of parasitism by A. ovale was 4.3% for larvae, and 10.1% for nymphs. The mean intensity of parasitism was 3.5 larvae/parasitized animal, and 1.6 nymphs/parasitized animal. One O. nigripes was found parasitized by both larva and nymph of A. ovale, and another O. nigripes was parasitized simultaneously by an I. schulzei nymph and five A. ovale nymphs. This research reports the following unpublished records: A. ovale on O. flavescens, O. nigripes and S. agouya; A. sculptum on S. agouya; and I. schulzei on O. flavescens and O. nigripes.


RESUMO: De maio a setembro de 2011, um total de 138 roedores silvestres da família Cricetidae foram coletados nos municípios de Anhembi, Bofete e Torre de Pedra, no estado de São Paulo. Todos os animais foram examinados quanto à presença de carrapatos, os quais foram coletados e identificados ao nível de espécie em laboratório, através de análises morfológicas (para adultos, ninfas e larvas) e por biologia molecular para confirmar estas análises, através do sequenciamento de um fragmento do gene 16S rDNA mitocondrial (para uma parte das larvas). Um total de 47 carrapatos (21 larvas e 26 ninfas) foi coletado dos roedores, sendo identificados como 21 larvas e 23 ninfas de Amblyomma ovale (coletados sobre Oligoryzomys nigripes, Oligoryzomys flavescens, Sooretamys agouya e Nectomys squamipes), duas ninfas de Ixodes schulzei (em O. nigripes e O. flavescens) e uma ninfa de Amblyomma sculptum em S. agouya. A prevalência geral de parasitismo por A. ovale foi de 4,3% e de 10,1% para larvas e ninfas, respectivamente. As intensidades médias de parasitismo foram de 3,5 larvas/animal parasitado e de 1,6 ninfas/animal parasitado. Um O. nigripes foi encontrado parasitado simultaneamente por larva e ninfa de A. ovale, e outro O. nigripes estava parasitado simultaneamente por uma ninfa de I. schulzei e cinco ninfas de A. ovale. O presente trabalho reporta os seguintes registros inéditos: A. ovale em O. flavescens, O. nigripes e S. agouya; A. sculptum em S. agouya; e I. schulzei em O. flavescens e O. nigripes.

4.
Biosci. j. (Online) ; 31(4): 1174-1182, july/aug. 2015.
Article in English | LILACS | ID: biblio-964586

ABSTRACT

The Kerodon rupestris are wild rodents bred in captivity in order to their conservation and development of researches. The aim of this study was describe the morphology of the tongue from eight animals by light microscopy and scanning electron microscopy, beyond macroscopic studies. The tongues of K. rupestris had 3.15 ± 0.28 cm of length; 1.0 ± 0.20 cm width at the root; 0.38 ± 0.10 cm width at the body; and, 1.10 ± 0.09 cm width at the apex. Thickness measurements were 0.50 ± 0.09 cm at the apex; 0.30 ± 0.10 cm at the body; and, 0.70 ± 0.10 cm at the root. The free apex had 0.50 ± 0.10 cm in length. The dorsal surface of the tongue contained the fungiform and filiform papillae at the apex and body; conical papillae and a pairs of vallate papillae at the root, and foliated papillae dorsolaterally positioned in the root region. The proper lamina of the dorsal surface of the tongue was composed by keratinized stratified squamous epithelium, in which lingual papillae were found; and, a rich network of skeletal striated muscle and non-modeled dense connective tissue, in which vessels, nerves, mucous and serous acini and their respective ducts were found. In summary, dorsal surface of the tongue of K. rupestris had filiform and conical papillae with mechanical function, and, fungiform, vallate and foliated papillae with taste buds responsible by gustatory function. Moreover, the tongue of these animals showed some peculiarities as the eminence on the apex in sagittal plane, which needs further studies.


Os Kerodon rupestris são roedores silvestres criados em cativeiro para sua conservação e desenvolvimento de pesquisas. O objetivo deste estudo foi descrever a língua destes animais por meio de microscopia de luz, microscopia eletrônica de varredura e estudos macroscópicos. As línguas de K. rupestris tinham 3,15 ± 0,28 cm de comprimento; 1,00 ± 0,20 cm de largura na raiz, 0,38 ± 0,10 cm de largura no corpo e 1,10 ± 0,09 de largura no ápice. As medidas de espessura foram 0,50 ± 0,09 cm no ápice, 0,30 ± 0,10 cm no corpo e 0,70 ± 0,10 cm na raíz. O ápice livre possuía 0,50 ± 0,10 cm de comprimento. A superfície dorsal da língua continha papilas fungiformes e filiformes no ápice e corpo; papilas cônicas e um par de papilas valadas na raiz; e, papilas foliadas posicionadas dorsolateralmente na região da raiz. A lâmina própria da superfície dorsal da língua estava composta por epitélio estratificado pavimentoso queratinizado, no qual as papilas linguais foram encontradas; e, uma rica rede de músculo estriado esquelético e tecido conjuntivo denso não modelado, no qual veias, nervos e ácinos serosos e mucosos e seus respectivos ductos foram encontrados. Em conclusão, a superfície da língua de K. rupestris apresentou papilas filiformes e cônicas com função mecânica e papilas fungiformes, valadas e foliadas com botões gustativos responsáveis pela função gustativa. Além disso, a língua desses animais apresentou ainda particularidades, como uma eminência no plano sagital do ápice que necessita de futuros estudos.


Subject(s)
Rodentia , Taste Buds , Microscopy, Electron , Digestive System , Mouth
5.
Pesqui. vet. bras ; 34(6): 593-598, jun. 2014. ilus
Article in Portuguese | LILACS | ID: lil-716352

ABSTRACT

A cutia (Dasyprocta sp.) é um roedor silvestre com distribuição mundial. Atualmente, além de importante papel ecológico que exerce, está sendo utilizada como modelo em experimento animal. Estudos sobre a morfologia destes animais são importantes porque podem ser uma alternativa para o estudo de diversos processos patológicos, além de contribuirem para a preservação da espécie. A laringe é um órgão localizado entre a faringe e a traqueia, no qual está envolvido nas funções de respiração, deglutição e fonação. O presente estudo propôs realizar uma descrição morfológica macroscópica e microscópica da laringe da cutia. Para tanto, foram utilizadas quinze cutias pertencentes ao Centro de Ciências Agrárias da Universidade Federal do Piauí e provenientes do Núcleo de Estudos e Preservação de Animais Silvestres com licença do IBAMA (Nº 02/08-618, CTF Nº 474064). Todos os animais foram identificados, promovida a sexagem e, posteriormente, a laringe acessada e dissecada sendo os fragmentos cartilagíneos encaminhados para rotina histológica e corados pelo método de hematoxilina-eosina. As lâminas obtidas foram visualizadas em microscopia óptica de luz e foto documentadas. A laringe da cutia apresenta cinco cartilagens, com ausência da cartilagem cuneiforme e presença da incisura caudal na cartilagem tireoide. O tecido epitelial da laringe varia de epitélio estratificado pavimentoso queratinizado à não queratinizado e ciliado com células caliciformes.


The agouti (Dasyprocta sp.) is a wild rodent with worldwide distribution. Besides its ecological role, agouti is used as a model in animal experimentation. Studies on the morphology of agouti are important because they can be an alternative for the study of various pathological processes and contribute to the preservation of the species. The larynx, located between the pharynx and trachea, is involved in the functions of breathing, swallowing and speech. This study aimed to perform a macroscopic and microscopic morphology of the larynx of agouti. To this end, we used fifteen agoutis belonging to the Centre of Agricultural Sciences, Federal University of Piauí, and from the Center for Study and Preservation of Wild Animals with license of IBAMA (No. 02/08-618, CTF No. 474064). All animals were identified according to their gender, the larynx was dissected and cartilaginous fragments were sent for routine histology and stained with hematoxylin-eosin. The slides obtained were viewed in light microscopy and photo documented. The larynx of agouti has five cartilages, without the cuneiform cartilage but presence of the thyroid cartilage notch. The epithelial tissue of the larynx varies from stratified squamous epithelium to keratinized and non-keratinized ciliated caliciforme cells.


Subject(s)
Animals , Male , Larynx/anatomy & histology , Microscopy/veterinary , Rodentia/anatomy & histology , Cricoid Cartilage , Epiglottis , Thyroid Cartilage
6.
Pesqui. vet. bras ; 31(7): 623-626, July 2011. ilus
Article in Portuguese | LILACS | ID: lil-600941

ABSTRACT

No estudo sobre a origem e ramificações das artérias mesentéricas cranial (AMCr) e caudal (AMCa) do mocó, foram utilizados 20 animais (18 machos e 2 fêmeas) de diferentes idades, que, após morte natural, foram dissecados rebatendo-se as paredes torácica e abdominal, pelo antímero esquerdo, expondo-se a aorta que foi então canulada em seu trajeto pré-diafragmático, procedendo-se a injeção de neoprene látex corado, no sentido caudal. A seguir, foram fixados em solução aquosa de formol a 10 por cento, durante 48 horas, e posteriormente dissecados. Os resultados mostraram que em 18 animais (90 por cento), a AMCr originou-se da aorta abdominal isoladamente, logo após a artéria celíaca, emitindo as artérias cólica média (CoM), pancreaticoduodenal caudal (PDC), duodenojejunal (DJ), jejunal (J) e ileocecocólica (ICeCo). Em um mocó (5 por cento), as AMCr e AC se originaram da aorta abdominal em um tronco comum. Neste caso, a AMCr originou às artérias CoM, PDC, ICeCo e J. Em uma observação (5 por cento), as artérias AMCr e AMCa surgiram em tronco comum. Neste animal, as artérias PDC, DJ, ICeCo, CoM e J foram originadas da AMCr, enquanto as aterias cólica esquerda (CoE) e retal cranial (ARCr) derivaram da AMCa. Dois animais (10 por cento) apresentaram como colaterais da AMCr as artérias CoM, PDC, DJ, J e o tronco ICeCo, que originou às artérias CoD e ileocecal (ICe). No que diz respeito a AMCa, nos 20 casos (100 por cento) originou as artérias CoE e RCr.


In this study about the origin and ramification of the cranial (CrMA) and caudal (CaMA) mesenteric collateral arteries of the rock cavy, 20 animals (18 males and 2 females) of different ages, originated from the Wild Animals Multiplication Center of the Universidade Federal Rural do Semi-Árido (Cemas/Ufersa), were used. After the natural death, the walls of the abdominal cavity of the animals, in the left antimere, were dissected to cannulate to the aorta in pre-diaphragmatic path. Then they were fixed in 10 percent formaline and conditioned in order to study their anatomy. The results showed that in 18 animals (90 percent) the CrMA arose, separately, of the abdominal aorta, soon after the celiac artery (CA), originating, by this time, the middle colic (MCo), caudal pancreaticduodenal (CPD), duodenojejune (DJ), jejune (J) and ileocecocolic (ICeCo) trunk from which derives the ileocecal (ICe) and the right colic (RCo) arteries. In one rock cavy (5 percent), the CrMA and CA originate from abdominal aorta in a common trunk. In this case the CrMA originated the CPD, MCo, ICeCo, and J. In one observation (5 percent) CrMA and CaMA appear in common trunk. In this animal, CPD, DJ, ICeCo, MCo and J arteries were originated of the CrMA, while the left colic (LCo) and rectal cranial (RCr) arteries were originated of the CaMA. Regarding the CaMA, in 20 cases (100,00 percent) it originates the LCo and the rectal cranial arteries.

7.
Rev. Soc. Bras. Med. Trop ; 43(3): 249-253, May-June 2010. ilus, tab
Article in English | LILACS | ID: lil-548518

ABSTRACT

INTRODUCTION: Chagas disease is caused by Trypanosoma cruzi. Wild and perianthropic mammals maintain the infection/transmission cycle, both in their natural habitat and in the peridomestic area. The aim of this paper was to present the results from a study on wild rodents in the central and northern regions of San Luis province, Argentina, in order to evaluate the prevalence of this infection. METHODS: Sherman traps were set up in capture areas located between latitudes 32º and 33º S, and longitudes 65º and 66º W. The captured rodents were taxonomically identified and hemoflagellates were isolated. Morphological, biometric and molecular studies and in vitro cultures were performed. Infection of laboratory animals and histological examination of the cardiac muscle and inoculation area were also carried out. Parasites were detected in circulating blood in Calomys musculinus, Graomys griseoflavus, Phyllotis darwini and Akodon molinae. The parasites were identified using biological criteria. Molecular PCR studies were performed on some isolates, which confirmed the characterization of these hemoflagellates as Trypanosoma cruzi. RESULTS AND CONCLUSIONS: Forty-four percent of the 25 isolates were identified as Trypanosoma cruzi, and the remaining 56 percent as Trypanosoma cruzi-like. These findings provide evidence that wild rats infected with Trypanosoma cruzi and Trypanosoma cruzi-like organisms are important in areas of low endemicity.


INTRODUÇÃO: A doença de Chagas é causada pelo Trypanosoma cruzi e os mamíferos periantrópicos e silvestres mantêm o ciclo de infecção/transmissão, tanto no ambiente natural, como no peridomicílio. O objetivo deste trabalho foi mostrar os resultados de um estudo de roedores silvestres do centro e norte da Província de San Luis, Argentina, para avaliar a prevalência da infecção. MÉTODOS: Estabeleceram-se lugares de caça com armadilhas tipo Sherman entre os 32º - 33º de latitude S e 65º - 66º de longitude W. Identificou-se taxonomicamente os roedores, isolou-se os hemoflagelados e fizeram-se estudos morfológicos, biométricos, moleculares, cultivo in vitro, infecção a animais de laboratório, histologia de músculo cardíaco e de zona de inoculação. Observou-se parasitas em sangue circulante: Calomys musculinus, Graomys griseoflavus, Phyllotis darwini e Akodon molinae. A identificação dos parasitas foi feita utilizando critérios biológicos e, em alguns, realizou estudos moleculares por PCR que confirmaram a caracterização desses hemoflagelados como Trypanosoma cruzi. RESULTADOS E CONCLUSÕES: Dos 25 isolados, 44 por cento foram identificados como Trypanosoma cruzi e 56 por cento como Trypanosoma cruzi like. Este achado nos induz a considerar a importância dos ratos do mato infectados com Trypanosoma cruzi y Trypanosoma cruzi like, em área de baixa endemicidade.


Subject(s)
Animals , Mice , Rats , Animals, Wild/parasitology , Disease Reservoirs/parasitology , Rodentia/parasitology , Trypanosoma cruzi/isolation & purification , Argentina , Chagas Disease/transmission , Mice, Inbred BALB C , Prevalence , Trypanosoma cruzi/classification , Trypanosoma cruzi/genetics
8.
Rev. biol. trop ; 57(1/2): 433-439, March-June 2009. graf
Article in English | LILACS | ID: lil-637731

ABSTRACT

Paternal behavior and testosterone plasma levels in the Volcano Mouse Neotomodon alstoni (Rodentia: Muridae). Although initially it was thought that testosterone inhibited the display of paternal behavior in males of rodents, it has been shown that in some species high testosterone levels are needed for exhibition of paternal care. In captivity, males of volcano Mouse (Neotomodon alstoni) provide pups the same care provided by the mother, with the exception of suckling. Here we measured plasmatic testosterone concentrations 10 days after mating, five and 20 days postpartum, and 10 days after males were isolated from their families in order to determine possible changes in this hormone, associated to the presence and age of pups. Males of volcano Mouse exhibited paternal behavior when their testosterone levels were relatively high. Although levels of this hormone did not change with the presence or pups age, males that invested more time in huddling showed higher testosterone levels. It is possible that in the volcano Mouse testosterone modulates paternal behavior indirectly, as in the California mouse. Rev. Biol. Trop. 57 (1-2): 433-439. Epub 2009 June 30.


Aunque inicialmente se pensaba que la testosterona inhibía el despliegue de conducta paterna en los machos de roedores, en algunas especies se ha demostrado que son necesarios niveles altos de testosterona para la exhibición de cuidados paternos. En cautiverio, los machos del ratón mexicano de los volcanes (Neotomodon alstoni), proporcionan a sus crías los mismos cuidados que la madre, con la excepción del amamantamiento. En este estudio se midieron los niveles plasmáticos de testosterona: en el día 10 del apareamiento, 5 y 20 días postparto, y 10 días después de aislar a los machos de sus familias, para establecer si los niveles de esta hormona cambian con relación a la presencia y edad de las crías. El ratón de los volcanes exhibió cuidados paternos cuando sus niveles de testosterona fueron relativamente altos, aunque los niveles de esta hormona no variaron significativamente con relación a la edad y presencia de las crías. Sin embargo, los machos que invirtieron más tiempo en abrigar a las crías, presentaron concentraciones relativamente más altas de testosterona. Es posible que en el ratón de los volcanes la testosterona regule la conducta paterna de manera indirecta, como ocurre con el ratón de California.


Subject(s)
Animals , Male , Mice , Muridae/blood , Muridae/psychology , Paternal Behavior , Testosterone/blood
9.
Journal of Bacteriology and Virology ; : 131-138, 2003.
Article in Korean | WPRIM | ID: wpr-20923

ABSTRACT

This study was carried out to determine the seropositivity for Orientia tsutsugamushi infection among wild rodents captured in farms and mountains of Korea. A total 965 wild rodents of 7 species and 48 Crocidura laciura were captured in farms and mountains of 6 province during 1997~2000 in Korea. Serological evidence for O. tsutsugamushi infection was obtained using O. tsutsugamushi antigens by indirect immunofluorescent antibody technique (IFA). Four hundred eighty four Apodemus agrariusus, 246 Apodemus peninsulae, 179 Eothenomys regulus, 44 Microtus fortis, 6 Micromys minutus, 5 Mus musculus, and 1 Cricetula triton were captured in Gyeonggi, Gangwon, Chungnam, Gyeongbuk, Gyeongnam, and Jeonbuk province. Among them, 59 (12.2%) A. agrariusus, 22 (8.9%) A. peninsulae, 12 (6.7%) E. regulus, 5 (11.4%) M. fortis and 1 (16.7%) M. minutus were IF antibody positive against O. tsutsugamushi Gilliam or Karp strain.


Subject(s)
Animals , Mice , Arvicolinae , Korea , Murinae , Neptune , Orientia tsutsugamushi , Rodentia
10.
Korean Journal of Medical Mycology ; : 147-154, 1998.
Article in Korean | WPRIM | ID: wpr-94319

ABSTRACT

BACKGROUND: Animals with zoophilic dermatophytes are important sources of dermatophytoses in man, because zoophilic dermatophytes are frequently transmitted directly or indirectly from domestic and wild animals. Trichophyton(T.) mentogrophytes has a wide range of hosts. Among these, rodents are well-known reservoirs in many other countries. In our country a few sporadic reports of incidence in experimental rats have been reported, but there is no study on the incidence in wild rodents in Korea. OBJECTIVE: The purpose of this study is to investigate the role of wild rodents as a host of dermatophytes and keratinophilic fungi in Korea. METHODS: Forty-nine wild rodents (Apodemus(A.) agrarius; 44, Crocidura spp; 5) were captured from 6 different regions of Kyungi-do with Sherman traps. Fungi were isolated by using the Mackenzie's brush technique. RESULTS: 1. Isolation rate of dermatophytes from wild rodents in Korea was 16.3%. 2. T. mentagrophytes was isolated from six A. agrarius (12.2%). Isolation rates from dorsum hairs, ventral hairs and feet were 4.5%, 9.1% and 9.1% respectively 3. Other dermatophytes and related keratinophilic fungi isolated were Chrysosporium spp.(65.3%), Scopulariopsis spp.(10.2%) and T. terrestre(4.1%). 4. Chrysosporium. was the most frequently isolated species from four out of six Kyonggi-do regions ranging 58.3%~83.3%. Scopulaiopsis was isolated the most in Kumgwang-myun Ansung-gun, and T. terrestre in Kimpo-gun. CONCLUSION: These results suggest that wild rodents, especially A. agrarius may be the important reservoirs of T. mentagrophytes and other keratinophilic fungi in Korea.


Subject(s)
Animals , Rats , Animals, Wild , Arthrodermataceae , Chrysosporium , Foot , Fungi , Hair , Incidence , Korea , Rodentia , Scopulariopsis , Tinea
11.
Korean Journal of Infectious Diseases ; : 443-449, 1998.
Article in Korean | WPRIM | ID: wpr-107533

ABSTRACT

BACKGROUND: This study was designed to determine the seropositivity for rickettsial infection among wild rodents captured in farms of Kyunggi province and in mountains of Kangwon province in Korea. METHODS: A total of 513 wild rodents of 5 species were captured in 1997~1998. Serological evidence for rickettsial infection was obtained using Orientia tsutsugamushi antigens by indirect immunofluorescent antibody technique(IFA). RESULTS: 168 Apodemus agrarius, 35 Microtus fortis, 3 Micromis minutus and 2 Eothenomys regulus were captured in Kyunggi province, of which 32 Apodemusagrarius, 4 Microtus fortis, 1 Micromis minutus were IF antibody positive against O. tsutsugamushi. 196 Apodemus agrarius, 77 Apodemus peninsulae and 29 Eothenomys regulus were captured in Kangwon province, of which 4 Apodemus agrarius and 5 Apodemus peninsulae were positive. CONCLUSION: Seropositivity rate against O. tsutsugamushi of wild rodents captured in Kyunggi and Kangwon provinces were 19.6% and 3.0% by IFA tests, respectively.


Subject(s)
Animals , Arvicolinae , Korea , Murinae , Orientia tsutsugamushi , Rodentia
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